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【优博微展】化工院彭瑞资:DNA纳米技术在生物医学中的应用研究

作者:   来源:   发布时间:2019-11-27 23:38   点击数:



论文题目:DNA纳米技术在生物医学中的应用研究

指导教师:谭蔚泓 教授

学科专业:化  学

培养学院:化学化工学院


伴随着纳米科技的迅猛发展,纳米材料的生物医学已引起了世界的广泛关注,我们选择广泛应用的DNA纳米材料作为构筑材料开展生物医学研究,围绕生物纳米技术开展生物医学方面的研究。

其中,细胞表面的成分相当丰富,其表面成分在细胞的成长、交流、增值和死亡中起着举足轻重的作用,这些细胞表面表达量不同的物质犹如每个人的脸部特征,识别不同的细胞表面也犹如“识人”。


我们研究了DNA纳米结构与细胞膜等生物界面的相互作用,对于构建膜表面化学生物传感器、提高药物递送效率具有重要的意义。然而,由于细胞对纳米材料内吞作用以及细胞膜结构的高度动态的特性,使得在活细胞的膜表面进行DNA纳米结构的组装调控研究变得十分困难。所以,我们开发了一种巨型仿生囊泡,构建了DNA纳米结构在该仿生膜表面的可逆调控。

实验以DNA自组装构建的DNA纳米棱柱为三维结构模型,通过DNA链杂交和置换反应实现了仿生囊泡表面DNA纳米棱柱的组装与解组装。由于该囊泡具有仿生细胞膜的特点和微米级的内腔室,通过功能化修饰,今后可望在生物分子动态调控、界面智能分子机器和人工细胞构建等领域得到广泛应用。

本论文针对前两个工作广泛应用的胆固醇-DNA做了考察,报道了在胆固醇修饰的DNA链的酸性条件下球形胶束和一维纳米棒的自组装。进一步的研究还表明:纳米棒是由胶束纳米结构分层组装而成。 可以通过改变溶液条件和胆固醇-DNA的设计来调节纳米棒组件的形态。胆固醇-DNA纳米结构在酸性条件下的自组装以及纳米棒与胶束之间关系的发现可为未来设计两亲性DNA嵌段共聚物的自组装提供新的策略。


主要学术成果

[1]Ruizi Peng, Xiaofang Zheng, Yifan Lyu, Liujun Xu, Xiaobing Zhang, Guoliang Ke, Qiaoling Liu, Changjun You, Shuangyan Huan and Weihong Tan. Engineering a 3D DNA-logic gate nanomachine for bispecific recognition and computing on target cell surfaces. Journal of the American Chemical Society, 2018, 140, 9793−9796

[2]Ruizi Peng, Huijing Wang, Yifan Lyu, Qiaoling Liu and Weihong Tan. Facile assembly/disassembly of DNA nanostructures anchored on cell-mimicking giant vesicles. Journal of the American Chemical Society, 2017, 139, 12410−12413.

[3]Ruizi Peng, Liujun Xu, Huijing Wang, Yifan Lyu, Dan Wang, Cheng Bi, Cheng Cui, Chunhai Fan, Qiaoling Liu, Weihong Tan. A DNA-based Artificial Molecular Signaling System that Mimics the Basic Elements of Reception, Transduction,and Response. (Submitted)

[4]Yifan Lv, Ruizi Peng, Yu Zhou, Xiaobing Zhang and Weihong Tan. Catalytic self-assembly of a DNA dendritic complex for efficient gene silencing. Chemical Communications, 2016, 52, 1413-1415. (co-first author)

[5]Yunlong Zhang, Ruizi Peng, Fengyuan Xu, Yonggang Ke. Hierarchical Self-assembly of Cholesterol-DNA Nanorods. Bioconjugate Chemistry. 2019, 30, 1845-1849.

[6]Xiaofang Zheng, Ruizi Peng, Xi Jiang, Yaya Wang, Shuai Xu, Guoliang Ke, Ting Fu, Qiaoling Liu, Shuangyan Huan, and Xiaobing Zhang. Fluorescence Resonance Energy Transfer-Based DNA Nanoprism with a Split Aptamer for Adenosine Triphosphate Sensing in Living Cells. Analytical Chemistry, 2017, 89, 10941−10947.

[7]Liang Cui, Ruizi Peng, Ting Fu, Xiaobing Zhang, Cuichen Wu, Huapei Chen, Hao Liang, Chaoyong James Yang, and Weihong Tan. Biostable L-DNAzyme for Sensing of Metal Ions in Biological Systems. Analytical Chemistry, 2016, 88, 1850–1855.

[8]Hui Liu, Qiuxia Yang, Ruizi Peng, Hailan Kuai, Yifan Lyu, Xiaoshu Pan, Qiaoling Liu, and Weihong Tan. Artificial signal feedback network mimicking cellular adaptivity. Journal of the American Chemical Society, 2019, 141, 6458–6461.

[9]Yifan Lv, Liang Cui, Ruizi Peng, Zilong Zhao, Liping Qiu, Huapei Chen,Cheng Jin, Xiao-Bing Zhang, and Weihong Tan. Entropy Beacon: A Hairpin-Free DNA Amplification Strategy for Efficient Detection of Nucleic Acids. Analytical Chemistry, 2015, 87, 11714–11720.

[10]Yifan Lyu, Yian Guo, Ren Cai, Ruizi Peng, Chengyi Hong, Xigao Chen, Weijia Hou, Xiaowei Li, Jie Tan, Yuxiu Zou, Xiaobing Zhang, Qiaoling Liu, Weihong Tan. Spherically Directed Synthesis and Enhanced Cellular Internalization of Metal-Crosslinked DNA Micelles. Chem, 2019, 5, 11714–11720.

[11]Hong-Min Meng, Hui Liu, Hailan Kuai, Ruizi Peng, Liuting Mo and Xiao-Bing Zhang. Aptamer-integrated DNA nanostructures for biosensing, bioimaging and cancer therapy. Chemical Society Reviews, 2016, 45, 2583-2602.

[12]Qiaoling Liu, Cheng Bi, Jiangling Li, Xuejiao Liu, Ruizi Peng, Cheng Jin, Yang Sun, Yifan Lyu, Hui Liu, Huijing Wang, Can Luo, Weihong Tan. Generating Giant Membrane Vesicles from Live Cells with Preserved Cellular Properties. Research 2019, 6523970.

[13]Xiaoyan Zhu, Longming Zhu, Hong-wen Liu, Xiaoxiao Hu, Ruizi Peng, Jing Zhang, Xiao-Bing Zhang and Weihong Tan. A two-photon fluorescent turn-on probe for imaging of SO2 derivatives in living cells and tissues. Analytica Chimica Acta, 2016, 937, 136-142.

[14]Ting Fu, Rui-Zi Peng, Qiao-Ling Liu. In situ DNA Assembly on the Surfaces of Nanosized Exosomes Based on Molecular Recognition. Acta Physico-Chimica Sinica, 2017, 33, 1083-1084.

图文来源:彭瑞资

审核:崔嘉媛 胡品 彭瑞资

责任编辑:研究生新媒体中心霍兴宇